GRK2 anticorps (C-Term)

N° du produit ABIN6655844

Détail du produit anti-GRK2 anticorps

Antigène: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GRK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée: Humain, Souris

Purification: This affinity purified antibody is directed against human GRK2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis of the sequence shows 100% reactivity to human, and 90% to rat, mouse, and golden hamster.

Immunogène:

Immunogen: Anti-GRK2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human GRK2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1000

Application Note: Anti-GRK2 Antibody has been tested in ELISA, Western Blot, and IHC. Expect a band at ~79.6 kDa in western blot using appropriate lysates. Positive control used: Human high grade lymphoma tissue in Immunohistochemistry and THP-1 lysate in western blot.

ELISA Dilution: 1:10,000-1:50,000

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur GRK2

Antigène: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Autre désignation: GRK2 (ADRBK1 Produits)

Synonymes: anticorps Adrbk-1, anticorps Bark-1, anticorps GRK2, anticorps betaARK1, anticorps BARK1, anticorps BETA-ARK1, anticorps GRK-2, anticorps Grk2, anticorps beta-adrenergic receptor kinase 1, anticorps G protein-coupled receptor kinase 2, anticorps Tsp_00497, anticorps grk2, anticorps Grk2, anticorps GRK2

Sujet:

Synonyms: Goat Anti-G Protein-Coupled Receptor Kinase 2 Antibody, Goat Anti-GRK2 Antibody, Beta-ARK-1, ADRBK1, BARK1, G-Protein Coupled Receptor Kinase 2, Adrenergic, Beta, Receptor Kinase 1, Adrenergic Beta Receptor Kinase 1, Beta-Adrenergic Receptor Kinase 1, EC 2.7.11.15, BETA-ARK1, BARK

Background: GRK2 (G Protein-Coupled Receptor Kinase 2) is a member of the G protein-coupled receptor kinase family of proteins. GRK2 Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. It is a key regulator of LPAR1 signaling. GRK2 competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. It desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. And positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. Anti-GRK2 Antibody is useful for researchers interested in Neuroscience, Cytokines & Growth Factors, and Stem Cell Research.

Gene Name: GRK2

ID gène: 156

NCBI Accession: NP_001610

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling