NOXA anticorps

N° du produit ABIN6655871

N° du produit (Fournisseur): 200-301-h98

Détail du produit anti-NOXA anticorps

Antigène: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NOXA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

N° du produit (Fournisseur): 200-301-h98

Fournisseur: Rockland

Fonction: NOXA Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Noxa from human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-Noxa Antibody was purified by Protein G chromatography.

Immunogène: Noxa Antibody was produced in mice prepared by repeated immunizations with the human protein Noxa.

Clone: 114C307-1

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 5 μg/mL

Western_Blot_Dilution: 1-2 μg/mL

Commentaires:

Anti-Noxa antibody is tested for use by WB, Flow, ICC/IF, IHC, and IHC-P. Expect a band approximately 6 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-NOXA (ABIN6655871)

Ichikawa, Alves, Pfeiffer, Langa, Hernández-Santana, Suzuki, Prehn, Engel, Henshall: "Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice." dans: Cell death & disease, Vol. 8, Issue 1, pp. e2556, (2017) (PubMed).

Détails sur NOXA

Antigène: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Autre désignation: Noxa (PMAIP1 Produits)

Sujet:

Synonyms: NOXA, Phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, Immediate-early-response protein APR, Protein Noxa

Background: Anti-Noxa antibody detects human Noxa. Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al. have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis. Anti-Noxa antibody is ideal for investigators involved in apaoptosis research.

Gene Name: PMAIP1

ID gène: 5366

NCBI Accession: NP_066950

UniProt: Q13794

Pathways: Signalisation p53, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus