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TJP1 anticorps

Rockland 600-401-gu7 TJP1 Reactivité: Humain WB, IHC, ELISA, IF, FACS, FM, MA Hôte: Lapin Polyclonal unconjugated
Rockland
N° du produit ABIN6655949
N° du produit (Fournisseur): 600-401-gu7
  • Antigène Voir toutes TJP1 Anticorps
    TJP1 (Tight Junction Protein 1 (TJP1))
    Reactivité
    • 59
    • 38
    • 37
    • 20
    • 18
    • 9
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 54
    • 3
    • 3
    • 1
    • 1
    Lapin
    Clonalité
    • 55
    • 7
    Polyclonal
    Conjugué
    • 38
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TJP1 est non-conjugé
    Application
    • 50
    • 19
    • 16
    • 14
    • 14
    • 13
    • 13
    • 12
    • 11
    • 8
    • 8
    • 3
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Multiplex Assay (MA)
    N° du produit (Fournisseur)
    600-401-gu7
    Fournisseur
    Rockland
    Fonction
    ZO-1 Antibody
    Réactivité croisée (Details)
    This affinity purified antibody is directed against human ZO-1.
    Purification
    This product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Stérilité
    Sterile filtered
    Immunogène
    Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
    Isotype
    IgG
    Top Product
    Discover our top product TJP1 Anticorps primaire
  • Indications d'application

    ELISA_Dilution: 10,000-1:50,000

    Immunohistochemistry_Dilution: 1:100-1:200

    Flow_Cytometry_Dilution: User Optimized

    IF_Microscopy_Dilution: 10 μg/mL

    Western_Blot_Dilution: 1:1000

    Commentaires

    Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    TJP1 (Tight Junction Protein 1 (TJP1))
    Autre désignation
    ZO-1 (TJP1 Produits)
    Sujet

    Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1

    Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.

    Gene Name: TJP1

    ID gène
    7082
    NCBI Accession
    NP_003248
    UniProt
    Q07157
    Pathways
    Carbohydrate Homeostasis, Cell-Cell Junction Organization
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