SMC1A anticorps (AA 951-962)

N° du produit ABIN6655981

Détail du produit anti-SMC1A anticorps

Antigène: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Épitope: AA 951-962

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SMC1A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Réactivité croisée: Humain, Souris

Purification: This Protein G Purified Mab antibody is directed against human SMC1 and is useful in determining its presence in various assays. This monoclonal anti-SMC1 antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.  Minimal reactivity is observed against the non-phosphorylated epitope.  Reactivity is observed against human and mouse SMC1.  Cross reactivity with SMC1 from other eukaryotic sources has not been tested.

Immunogène:

Immunogen: This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.

Immunogen Type: Peptide

Clone: 5D11-G5

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Protein G Purified Mab anti-SMC1 was tested by ELISA, immunohistochemistry and western blotting against native protein. The antibody reacts with SMC1 from irradiated human and mouse cells.  A 160 kDa band corresponding to phosphorylated human SMC1 is noted in gamma irradiated human and mouse lysates.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:100 - 1:2,000

IF Microscopy Dilution: 2.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Références pour anticorps anti-SMC1A (ABIN6655981)

Callén, Jankovic, Wong, Zha, Chen, Difilippantonio, Di Virgilio, Heidkamp, Alt, Nussenzweig, Nussenzweig: "Essential role for DNA-PKcs in DNA double-strand break repair and apoptosis in ATM-deficient lymphocytes." dans: Molecular cell, Vol. 34, Issue 3, pp. 285-97, (2009) (PubMed).

Pusapati, Rounbehler, Hong, Powers, Yan, Kiguchi, McArthur, Wong, Johnson: "ATM promotes apoptosis and suppresses tumorigenesis in response to Myc." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1446-51, (2006) (PubMed).

Kitagawa, Bakkenist, McKinnon, Kastan: "Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway." dans: Genes & development, Vol. 18, Issue 12, pp. 1423-38, (2004) (PubMed).

Détails sur SMC1A

Antigène: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Autre désignation: SMC1 (SMC1A Produits)

Synonymes: anticorps CDLS2, anticorps DXS423E, anticorps SB1.8, anticorps SMC1, anticorps SMC1L1, anticorps SMC1alpha, anticorps SMCB, anticorps SMC-1A, anticorps NV15570, anticorps 5830426I24Rik, anticorps Sb1.8, anticorps Smc1, anticorps Smc1alpha, anticorps Smc1l1, anticorps Smcb, anticorps mKIAA0178, anticorps smc1, anticorps smc1a, anticorps smc1l1, anticorps xSMC1, anticorps smc1aa, anticorps structural maintenance of chromosomes 1A, anticorps structural maintenance of chromosomes 1A b, anticorps structural maintenance of chromosomes 1A L homeolog, anticorps SMC1A, anticorps smc1a, anticorps Smc1a, anticorps smc1ab, anticorps smc1a.L

Sujet:

Synonyms: mouse anti-SMC1 pS957 antibody, Structural maintenance of chromosomes protein 1B antibody, SMC1beta protein antibody, SMC1B antibody, SMC1L2 antibody

Background: Structural maintenance of chromosomes (SMC) proteins play important roles in sister chromatid cohesion, chromosome condensation, sex-chromosome dosage compensation, and DNA recombination and repair (DNA damage). Protein complexes containing heterodimers of the SMC1 and SMC3 proteins have been implicated specifically in both sister chromatid cohesion and DNA recombination.  ATM, a protein kinase belonging to the phosphatidylinositol 3-kinase family that regulates cell cycle checkpoints and DNA recombination and repair, phosphorylates SMC1 protein after ionizing irradiation. ATM protein kinase phosphorylates SMC1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint.  Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM protein kinase substrates NBS1 and BRCA1. These same sites in SMC1 are phosphorylated after treatment with UV irradiation or hydroxyurea in an ATM-independent manner, thus demonstrating that another kinase must be involved in responses to these cellular stresses. Yeast containing hypomorphic mutations in SMC1 and human cells overexpressing SMC1 mutated at both of these phosphorylation sites exhibit decreased survival following ionizing irradiation. These results demonstrate that SMC1 participates in cellular responses to DNA damage and link SMC1 to the ATM protein kinase signal transduction pathway.

Gene Name: SMC1B

ID gène: 27127

UniProt: Q8NDV3

Pathways: Stem Cell Maintenance