UBE2M anticorps (AA 169-180)

N° du produit ABIN6656076

Détail du produit anti-UBE2M anticorps

Antigène: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Épitope: AA 169-180

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE2M est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Chimpanzé, Chien, Grenouille, Humain, Souris, Rat (Rattus)

Purification: UBC12 Antibody is produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human UBC12 protein. However, 100% homology is on record for this protein from human, chimpanzee, frog, mouse, rat and dog as indicated by BLAST analysis.  Reactivity with UBC12 proteins from other sources is not known.

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 169-180 of Human UBC12 protein.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: UBC12 Antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to UBC12 by western blotting in the appropriate cell lysate or extract.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Références pour anticorps anti-UBE2M (ABIN6656076)

Liu, Reitsma, Mamrosh, Zhang, Straube, Deshaies: "Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression." dans: Molecular cell, Vol. 69, Issue 5, pp. 773-786.e6, (2019) (PubMed).

Kolokoltsova, Domina, Kolokoltsov, Davey, Weaver, Watowich: "Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling." dans: Virology, Vol. 377, Issue 1, pp. 133-42, (2008) (PubMed).

Détails sur UBE2M

Antigène: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Autre désignation: UBC12 (ube2m Produits)

Synonymes: anticorps DDBDRAFT_0204633, anticorps DDBDRAFT_0238042, anticorps DDB_0204633, anticorps DDB_0238042, anticorps UBC-RS2, anticorps UBC12, anticorps hUbc12, anticorps 2510040H03Rik, anticorps Ubc-rs2, anticorps ubiquitin conjugating enzyme E2M, anticorps ubiquitin-conjugating enzyme E2M, anticorps ubiquitin conjugating enzyme E2 M, anticorps NEDD8-conjugating enzyme, anticorps ubiquitin conjugating enzyme E2M S homeolog, anticorps ube2m, anticorps LOC692934, anticorps UBE2M, anticorps Ube2m, anticorps ube2m.S

Sujet:

Synonyms: rabbit anti-UBC12 antibody, UBC-12, UBC 12, NEDD-8, NEDD 8, NEDD8 carrier protein antibody, NEDD8 conjugating enzyme Ubc12 antibody, NEDD8 protein ligase antibody, UBE2M antibody, Ubiquitin conjugating enzyme E2 M antibody

Background: UBC12 (also known as Ubiquitin-conjugating enzyme E2M, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, and Nedd8-conjugating enzyme Ubc12) is a member of the E2 ubiquitin-conjugating enzyme family.  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBC12 is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/cullin.

Gene Name: UBE2M

ID gène: 9040, 4507791

UniProt: P61081