PIK3CD anticorps (C-Term)

N° du produit ABIN6657601

Détail du produit anti-PIK3CD anticorps

Antigène: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Épitope: C-Term

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIK3CD est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Humain, Souris

Réactivité croisée (Details): Cross reactivity is expected against the human protein as the sequence of the immunogen is 100 % identical in both human and mouseCross reactivity is also expected against rat PI3K p110d.

Purification: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.  Reactivity occurs against mouse PI3K p110d subunit. Cross reactivity is expected against the human protein as the sequence of the immunogen is 100% identical in both human and mouse. Cross reactivity is also expected against rat PI3K p110d.

Immunogène:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of mouse PI3K p110d. This sequence is identical in both mouse and human.

Immunogen Type: Peptide

Information d'application

Indications d'application:

Application Note: This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.  A mouse whole cell splenic lysate is suitable for use as a positive control.

ELISA Dilution: 1:4,000 - 1:20,000

Western Blot Dilution: 1:5,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Références pour anticorps anti-PIK3CD (ABIN6657601)

Jou, Carpino, Takahashi, Piekorz, Chao, Carpino, Wang, Ihle: "Essential, nonredundant role for the phosphoinositide 3-kinase p110delta in signaling by the B-cell receptor complex." dans: Molecular and cellular biology, Vol. 22, Issue 24, pp. 8580-91, (2003) (PubMed).

Détails sur PIK3CD

Antigène: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Autre désignation: PI3-kinase p110 delta (PIK3CD Produits)

Synonymes: anticorps 2410099E07Rik, anticorps 2610208K16Rik, anticorps AW545373, anticorps p110delta, anticorps P110DELTA, anticorps PI3K, anticorps p110D, anticorps phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta, anticorps Pik3cd, anticorps PIK3CD

Sujet:

Synonyms: rabbit anti-PI3-kinase p110 delta Antibody, p110D antibody, p110delta antibody, Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody, PI3K antibody, PIK3CD antibody

Background: Phosphoinositide 3-kinases (PI3Ks) generate 3-phosphoinositide lipids in cell membranes.  A variety of intracellular target proteins interact with these lipids via specific lipid-binding modules and, as a consequence, undergo changes in their localization and/or activity. In this way, PI3Ks participate in the regulation of mitogenesis, differentiation, survival, intracellular vesicular transport, cytoskeletal reorganization, and motility.  Tyrosine kinases and Ras use PI3Ks as essential intracellular signal relay molecules. PI3Ks are heterodimeric enzymes consisting of a regulatory subunit in complex with a p110 catalytic subunit. Mammmals have genes encoding three distinct catalytic subunits (p110α, p110ß, and p110δ and three regulatory subunits (p85α, p85ß, and p55δ). All of the p110 isoforms are capable of interacting with each type of regulatory subunit. They are also similarly recruited to phosphotyrosine complexes and have, at least in vitro, the same lipid substrate specificity. However, it is becoming increasingly clear that PI3K isoforms differ in their interaction with Ras and regulation of lipid kinase activity, and in their protein kinase activities. Several groups have provided evidence that p110 isoforms have nonredundant functions in the regulation of cell proliferation, survival, actin cytoskeleton reorganization, and migration downstream of given receptors. This antibody is specific for the carboxy teminal end of p110d that is expressed predominantly in leukocytes.

Gene Name: PIK3CD

ID gène: 18707

UniProt: O35904

Pathways: BCR Signaling, L'effet Warburg