BAT3 anticorps (C-Term)

N° du produit ABIN6657746

N° du produit (Fournisseur): 100-401-b63

Détail du produit anti-BAT3 anticorps

Antigène: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Épitope: C-Term

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BAT3 est non-conjugé

Application: Western Blotting (WB), ELISA

N° du produit (Fournisseur): 100-401-b63

Fournisseur: Rockland

Fonction: Scythe/Bat3 Antibody

Purification: This product was prepared from monospecific antiserum by addition of Sodium azide to 0.01 % .

Stérilité: Sterile filtered

Immunogène: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/BAT3.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: User Optimized

Western_Blot_Dilution: 1:5,000 to 1:8000

Other: User Optimized

Commentaires:

This polyclonal antibody has been tested for use in western blotting and is specific for mouse Scythe/Bat3 and its isoforms. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands, with the predominant at approximately 200kDa in size corresponding to Scythe/BAT3 by western blotting in the appropriate cell lysate or extract. In western blot analysis of endogenous protein, multiple bands of Scythe/BAT3 may appear corresponding to variable amount of splice isoforms present.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-BAT3 (ABIN6657746)

Desmots, Russell, Michel, McKinnon: "Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis." dans: The Journal of biological chemistry, Vol. 283, Issue 6, pp. 3264-71, (2008) (PubMed).

Desmots, Russell, Lee, Boyd, McKinnon: "The reaper-binding protein scythe modulates apoptosis and proliferation during mammalian development." dans: Molecular and cellular biology, Vol. 25, Issue 23, pp. 10329-37, (2005) (PubMed).

Détails sur BAT3

Antigène: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Autre désignation: Scythe/Bat3 (BAT3 Produits)

Sujet:

Synonyms: rabbit anti-Bat3 Antibody, rabbit anti-Scythe Antibody, Human Leukocyte Antigen-B-Associated Transcript 3, HLA-B-associated transcript 3, BAT3, Large proline-rich protein BAT3

Background: Scythe protein (also known as BAT3 and HLA-B-associated transcript 3) is an apoptotic regulator that is highly conserved in eukaryotes and contains a ubiquitin-like domain near its N-terminus. It binds Reaper, a potent apoptotic inducer, as well as Hid and Grim. Scythe and Reaper are thought to signal apoptosis, in part through regulating the folding and activity of apoptotic signaling molecules. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis. It interacts with transforming growth factor-beta (TGF-beta) receptors and enhances TGF-beta1-induced type I collagen expression in mesangial cells. With SET1A, Scythe forms a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. Scythe is essential for p300-mediated acetylation of p53. Scythe/BAT3 is identified as a critical regulator of Hsp70-2 in spermatogenesis, thereby providing a possible molecular target in idiopathic male infertility. The human Scythe/BAT3 ortholog in rodents is predominantly and developmentally expressed in testis.

Gene Name: BAT3

ID gène: 224727, 33147082

UniProt: Q9Z1R2