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DAB1 anticorps (AA 400-555)

Rockland 100-401-225 DAB1 Reactivité: Souris WB, ELISA, IHC, IP, FM Hôte: Lapin Polyclonal unconjugated
Rockland
N° du produit ABIN6657863
N° du produit (Fournisseur): 100-401-225
  • Antigène Voir toutes DAB1 Anticorps
    DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))
    Épitope
    • 15
    • 15
    • 15
    • 7
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 400-555
    Reactivité
    • 53
    • 51
    • 50
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Souris
    Hôte
    • 84
    • 3
    • 3
    Lapin
    Clonalité
    • 89
    • 1
    Polyclonal
    Conjugué
    • 41
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    Cet anticorp DAB1 est non-conjugé
    Application
    • 68
    • 41
    • 41
    • 32
    • 22
    • 16
    • 11
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
    N° du produit (Fournisseur)
    100-401-225
    Fournisseur
    Rockland
    Fonction
    Dab1 Antibody
    Réactivité croisée (Details)
    This antibody is directed against Dab1 from mouse.
    Purification
    Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.
    Stérilité
    Sterile filtered
    Immunogène
    This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555.
    Isotype
    IgG
    Top Product
    Discover our top product DAB1 Anticorps primaire
  • Indications d'application

    Immunoprecipitation_Dilution: 1 μL per 500 μg

    ELISA_Dilution: 1:10,000 - 1:50,000

    Immunohistochemistry_Dilution: 1:5,000

    IF_Microscopy_Dilution: User Optimized

    Western_Blot_Dilution: 1:5,000

    Other: User Optimized

    Commentaires

    Suggested Applications: Biochemical Assay, IF, Multiplex
    Anti-Dab1 antibody has been tested by western blot and immunohistochemistry and is suitable for the detection of Dab1 by immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate.  For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature.  For immunoprecipitation use 1 μl of antiserum per 500 μg of brain lysate.  Perform immunoprecipitation at 4°C for 2 h.  For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 μg/ml aprotinin and 10 μg/ml leupeptin.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Wang, Lei, Wang, Yu, He, Zhao, Hu, Xu, Jin, Gu, Guo, Yang, Gao, Wang: "The ZSWIM8 ubiquitin ligase regulates neurodevelopment by guarding the protein quality of intrinsically disordered Dab1." dans: Cerebral cortex (New York, N.Y. : 1991), Vol. 33, Issue 7, pp. 3866-3881, (2023) (PubMed).

    Reyes, Hino, Canales, Dickson, La Torre, Simó: "The E3 Ubiquitin Ligase CRL5 Regulates Dentate Gyrus Morphogenesis, Adult Neurogenesis, and Animal Behavior." dans: Frontiers in neuroscience, Vol. 16, pp. 908719, (2022) (PubMed).

    Meseke, Neumüller, Brunne, Li, Anstötz, Pohlkamp, Rogalla, Herz, Rune, Bender: "Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin." dans: eNeuro, Vol. 5, Issue 5, (2019) (PubMed).

    Fairchild, Hino, Han, Miltner, Peinado Allina, Brown, Burns, La Torre, Simó: "RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion." dans: Development (Cambridge, England), Vol. 145, Issue 3, (2018) (PubMed).

    O'Brien, Hirano, Buttermore, Bhat, Peles: "Localization of the paranodal protein Caspr in the mammalian retina." dans: Molecular vision, Vol. 16, pp. 1854-63, (2011) (PubMed).

    de la Maza, Olivares, Hirsch, Sierralta, Gattás, Barrera, Bunout, Leiva, Fernández: "Weight increase and overweight are associated with DNA oxidative damage in skeletal muscle." dans: Clinical nutrition (Edinburgh, Scotland), Vol. 25, Issue 6, pp. 968-76, (2007) (PubMed).

    Katyal, Gao, Monckton, Glubrecht, Godbout: "Hierarchical disabled-1 tyrosine phosphorylation in Src family kinase activation and neurite formation." dans: Journal of molecular biology, Vol. 368, Issue 2, pp. 349-64, (2007) (PubMed).

    Hanzlicek, Peachey, Grimm, Hagstrom, Ball: "Probing inner retinal circuits in the rod pathway: a comparison of c-fos activation in mutant mice." dans: NULL, Vol. 21, Issue 6, pp. 873-81, (2005) (PubMed).

    Bramblett, Pennesi, Wu, Tsai: "The transcription factor Bhlhb4 is required for rod bipolar cell maturation." dans: Neuron, Vol. 43, Issue 6, pp. 779-93, (2004) (PubMed).

    Keshvara, Magdaleno, Benhayon, Curran: "Cyclin-dependent kinase 5 phosphorylates disabled 1 independently of Reelin signaling." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 12, pp. 4869-77, (2002) (PubMed).

    Sheldon, Rice, DArcangelo, Yoneshima, Nakajima, Mikoshiba, Howell, Cooper, Goldowitz, Curran: "Scrambler and yotari disrupt the disabled gene and produce a reeler-like phenotype in mice." dans: Nature, Vol. 389, Issue 6652, pp. 730-3, (1997) (PubMed).

  • Antigène
    DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))
    Autre désignation
    Dab1 (DAB1 Produits)
    Sujet

    Synonyms: rabbit anti-Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody

    Background: Anti-Dab1 Antibody recognizes Dab1 that is a phosphoprotein encoded by the mouse gene dab and is related to the Drosophila gene 'disabled'.  Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes.  Dab1 binds to non-receptor tyrosine kinases and plays an important role in brain development.  Dab1 is expressed in neuronal populations exposed to reelin, and it functions as a signaling molecule that regulates cell positioning in the developing brain.  Cloning of human Dab1 and sequence determinations suggest a 96 % identity to the mouse sequence.  Dab1 binds to cytoplasmic regions of very low density lipoprotein receptors (VLDLR), apolipoprotein E receptor-2 (ApoER2) and the Amyloid Precursor Protein (APP) family of proteins.  Dab1 accumulates in ectopic neurons from mice lacking Reelin or both VLDLR and ApoER2.  In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1.

    Gene Name: DAB1

    ID gène
    13131
    NCBI Accession
    NP_034144
    UniProt
    P97318
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