SHANK1 anticorps (Internal Region)

N° du produit ABIN6658104

Détail du produit anti-SHANK1 anticorps

Antigène: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Épitope: Internal Region

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SHANK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: Anti-SHANK1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with SHANK1 from human, mouse, and rat based on 100% homology with the immunizing sequence. No cross-reactivity against Shank2 or Shank3. Cross-reactivity with Shank1 from other sources has not been determined. Scaffolds research.

Immunogène:

Immunogen: SHANK1 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an internal region of (SH3/PDZ domains) of rat Shank1.

Immunogen Type: Recombinant Protein

Clone: S22-21

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-SHANK1 Antibody is suitable for use in WB and IHC. Expect a band approximately ~190-220 kDa (alternative splice variants) on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur SHANK1

Antigène: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Autre désignation: SHANK1 (SHANK1 Produits)

Synonymes: anticorps SPANK-1, anticorps SSTRIP, anticorps synamon, anticorps Shank1a, anticorps Spank1, anticorps Sstrip, anticorps SH3 and multiple ankyrin repeat domains 1, anticorps SHANK1, anticorps Shank1

Sujet:

Synonyms: Spank1, Sstrip, Shank1a, Shank1, SH3 and multiple ankyrin repeat domains protein 1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank1 specifically might be relevant to human autism spectrum disorders, due to its differential role in specific cognitive processes, along with its importance for synapse structure and function in vivo.

Gene Name: Spank1

ID gène: 78957

NCBI Accession: NP_113939

UniProt: Q9WV48

Pathways: Synaptic Membrane, Maintenance of Protein Location