INA anticorps

N° du produit ABIN6658126

N° du produit (Fournisseur): 200-901-d04

Détail du produit anti-INA anticorps

Antigène: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivité: Rat

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp INA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM), Multiplex Assay (MA)

N° du produit (Fournisseur): 200-901-d04

Fournisseur: Rockland

Fonction: Alpha Internexin Antibody

Réactivité croisée (Details): Anti-Alpha Internexin Antibody is directed against rat Alpha Internexin proteins.

Purification: The antibody was purified from chicken eggs as an IgY fraction.

Immunogène: Anti-Alpha Internexin Antibody was produced by repeated immunizations with recombinant rat alpha internexin expressed in E. coli.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:50,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:5000

Commentaires:

Anti-Alpha Internexin antibody is tested for use in Western Blotting and ICC, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 66 kDa in size corresponding to the alpha internexin protein in Western blots in the appropriate cell lysate or extract. Minor bands at ~150k are probably covalent dimers and bands at ~50k represent alpha-internexin breakdown products.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur INA

Antigène: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Autre désignation: Alpha Internexin (INA Produits)

Sujet:

Synonyms: Alpha INX, internexin, NF-66, NEF5

Background: Anti-Alpha Internexin antibody recognizes alpha-internexin which is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits. Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals. Anti-Alpha Internexin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: INA

ID gène: 24503

UniProt: P23565