TRAC anticorps (AA 1-114)

N° du produit ABIN6719654

Détail du produit anti-TRAC anticorps

Antigène: TRAC (T Cell Receptor alpha Constant (TRAC))

Épitope: AA 1-114

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRAC est non-conjugé

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Fonction: Rabbit IgG polyclonal antibody for TCR alpha detection. Tested with WB, FCM, Direct ELISA in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for TCR alpha detection. Tested with WB, FCM, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human TCR alpha recombinant protein (Position: I1-L114).

Isotype: IgG

Information d'application

Indications d'application:

Application details: Western blot|0.1-0.5 μg/mL Flow Cytometry|1-3 μg/1x106 cells Direct ELISA|0.1-0.5 μg/mL

Commentaires:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur TRAC

Antigène: TRAC (T Cell Receptor alpha Constant (TRAC))

Autre désignation: TRAC (TRAC Produits)

Synonymes: anticorps TCRA, anticorps Gm16914, anticorps Tcra, anticorps Tcra-C, anticorps T-cell receptor alpha constant, anticorps T cell receptor alpha constant, anticorps TRAC, anticorps Trac

Sujet:

Synonyms: T-cell receptor alpha chain C region, TRAC, TCRA

Background: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes.

ID gène: 28755

UniProt: P01848