Splicing Factor 1 anticorps (AA 517-566)

N° du produit ABIN6736753

Détail du produit anti-Splicing Factor 1 anticorps

Antigène: Splicing Factor 1 (SF1)

Épitope: AA 517-566

Reactivité: Humain, Souris, Rat, Chien, Lapin, Boeuf (Vache), Cheval, Cobaye, Singe, Porc, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Splicing Factor 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human SF1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa517-566 of human SF1 (Q15637, NP_004621). Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Armadillo (100%), Gibbon, Slime mold (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur Splicing Factor 1

Antigène: Splicing Factor 1 (SF1)

Autre désignation: SF1 (SF1 Produits)

Sujet: Name/Gene ID: SF1

Synonyms: SF1, D11S636, MBBP, ZCCHC25, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, BBP, ZNF162, Splicing factor 1, ZFM1

ID gène: 7536

NCBI Accession: NP_004621

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location