GATA6 anticorps (C-Term)

N° du produit ABIN462170

Détail du produit anti-GATA6 anticorps

Antigène: GATA6 (GATA Binding Protein 6 (GATA6))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cobaye, Cheval, Roussette (Chauve-souris), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GATA6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human GATA6

Homologie: Percent identity by BLAST analysis: Human, Horse (100%) Sheep, Rabbit, Pig (92%) Rat, Bovine (85%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human GATA6 (Q92908, NP_005248). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bat, Horse (100%), Sheep, Rabbit, Pig (92%), Rat, Panda, Bovine (85%), Guinea pig (84%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IF

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur GATA6

Antigène: GATA6 (GATA Binding Protein 6 (GATA6))

Autre désignation: GATA6 (GATA6 Produits)

Synonymes: anticorps AA410133, anticorps GATA-6, anticorps XGATA-6, anticorps XGATA6, anticorps gata6a, anticorps gata6b, anticorps pnr, anticorps tGata6, anticorps gata6, anticorps gata-6, anticorps GATA-5, anticorps cb603, anticorps GATA binding protein 6, anticorps GATA binding protein 6 S homeolog, anticorps GATA binding protein 6 L homeolog, anticorps GATA6, anticorps Gata6, anticorps gata6, anticorps gata6.S, anticorps gata6.L

Sujet: Name/Gene ID: GATA6

Synonyms: GATA6, GATA-binding factor 6, GATA-binding protein 6, GATA binding protein 6, Transcription factor GATA-6

ID gène: 2627

NCBI Accession: NP_005248

UniProt: Q92908