LIN28A anticorps (N-Term)
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- Antigène Voir toutes LIN28A Anticorps
- LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))
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Épitope
- N-Term
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Reactivité
- Humain, Porc, Boeuf (Vache), Chien, Mouton
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp LIN28A est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human LIN28A / LIN28
- Homologie
- Percent identity by BLAST analysis: Dog, Bovine, Pig (100%) Rat, Sheep (85%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic peptide from N-Terminus of human LIN28A (Q9H9Z2, NP_078950). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Panda, Dog, Cat, Bovine, Pig (100%), Rabbit (92%), Monkey, Mouse, Rat, Elephant (90%), Sheep, Guinea pig (85%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product LIN28A Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Antigène
- LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))
- Autre désignation
- LIN28A / LIN28 (LIN28A Produits)
- Synonymes
- anticorps CSDD1, anticorps LIN-28, anticorps LIN28, anticorps ZCCHC1, anticorps lin-28A, anticorps AL024421, anticorps ENSMUSG00000070700, anticorps Gm10299, anticorps Lin-28, anticorps Lin28, anticorps Tex17, anticorps csdd1, anticorps lin-28, anticorps lin28, anticorps zcchc1, anticorps sb:cb650, anticorps zgc:55584, anticorps RGD1566408, anticorps lin-28 homolog A, anticorps lin-28 homolog A (C. elegans), anticorps lin-28 homolog A S homeolog, anticorps LIN28A, anticorps Lin28a, anticorps lin28a.S, anticorps lin28a
- Sujet
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Name/Gene ID: LIN28A
Synonyms: LIN28A, CSDD1, Lin-28 homolog (C. elegans), LIN-28, Lin-28A, LIN28, Protein lin-28 homolog A, Lin-28 homolog A (C. elegans), ZCCHC1, RNA-binding protein LIN-28 - ID gène
- 79727
- NCBI Accession
- NP_078950
- UniProt
- Q9H9Z2
- Pathways
- Stem Cell Maintenance
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