ZNF165 anticorps (AA 216-265)

N° du produit ABIN6741560

Détail du produit anti-ZNF165 anticorps

Antigène: ZNF165 (Zinc Finger Protein 165 (ZNF165))

Épitope: AA 216-265

Reactivité: Humain, Porc, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ZNF165 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human ZNF165

Homologie: Percent identity by BLAST analysis: Human (100%) Pig (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa216-265 of human ZNF165 (P49910, NP_003438). Percent identity by BLAST analysis: Human, Monkey (100%), Gorilla, Gibbon (92%), Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur ZNF165

Antigène: ZNF165 (Zinc Finger Protein 165 (ZNF165))

Autre désignation: ZNF165 (ZNF165 Produits)

Synonymes: anticorps CT53, anticorps LD65, anticorps ZSCAN7, anticorps ZSCAN16, anticorps MGC140342, anticorps zinc finger protein 165, anticorps ZNF165

Sujet: Name/Gene ID: ZNF165
Family: Zinc Finger

Synonyms: ZNF165, Cancer/testis antigen 53, CT53, LD65, ZPF165, Zinc finger protein 165, ZSCAN7

ID gène: 7718

NCBI Accession: NP_003438

UniProt: P49910