BCL11A anticorps (N-Term)

N° du produit ABIN6741578

Détail du produit anti-BCL11A anticorps

Antigène: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Porc, Poisson zèbre (Danio rerio), Poulet, Roussette (Chauve-souris), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BCL11A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human BCL11A

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Chicken, Zebrafish (100%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human BCL11A (Q9H165, NP_075044). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Chicken, Platypus, Zebrafish (100%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur BCL11A

Antigène: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Autre désignation: BCL11A (BCL11A Produits)

Synonymes: anticorps 2810047E18Rik, anticorps BCL-11A, anticorps Ctip1, anticorps D930021L15Rik, anticorps Evi9, anticorps Evi9a, anticorps Evi9b, anticorps Evi9c, anticorps mKIAA1809, anticorps BCL11A-L, anticorps BCL11A-S, anticorps BCL11A-XL, anticorps BCL11a-M, anticorps CTIP1, anticorps EVI9, anticorps HBFQTL5, anticorps ZNF856, anticorps bcl11a, anticorps wu:fi09f10, anticorps zgc:136898, anticorps B cell CLL/lymphoma 11A (zinc finger protein), anticorps B-cell CLL/lymphoma 11A, anticorps B-cell CLL/lymphoma 11A L homeolog, anticorps B-cell CLL/lymphoma 11Aa, anticorps Bcl11a, anticorps BCL11A, anticorps bcl11a.L, anticorps bcl11aa

Sujet: Name/Gene ID: BCL11A
Family: Cancer

Synonyms: BCL11A, B-cell CLL/lymphoma 11A, BCL11A-L, BCL11A-XL, BCL11a-M, B-cell lymphoma/leukemia 11A, BCL-11A, BCL11A-S, CTIP1, COUP-TF-interacting protein 1, HBFQTL5, EVI-9, KIAA1809, ZNF856, C2H2-type zinc finger protein, EVI9, Zinc finger protein 856

ID gène: 53335

NCBI Accession: NP_075044

UniProt: Q9H165

Pathways: Regulation of Cell Size