TRIM68 anticorps (AA 395-444)

N° du produit ABIN6741579

Détail du produit anti-TRIM68 anticorps

Antigène: TRIM68 (Tripartite Motif Containing 68 (TRIM68))

Épitope: AA 395-444

Reactivité: Humain, Souris, Boeuf (Vache), Cheval, Porc, Chien, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM68 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human TRIM68

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Horse, Pig (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa395-444 of human TRIM68 (Q6AZZ1, NP_060543). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Horse, Pig (100%), Galago, Bat (92%), Opossum (84%), Turkey, Chicken (80%), Rat (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile dH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur TRIM68

Antigène: TRIM68 (Tripartite Motif Containing 68 (TRIM68))

Autre désignation: TRIM68 (TRIM68 Produits)

Synonymes: anticorps GC109, anticorps RNF137, anticorps SS-56, anticorps SS56, anticorps AI894158, anticorps F730114J12Rik, anticorps Rnf137, anticorps TRIM68, anticorps tripartite motif containing 68, anticorps tripartite motif-containing 68, anticorps E3 ubiquitin-protein ligase TRIM68, anticorps TRIM68, anticorps Trim68, anticorps LOC100053519

Sujet: Name/Gene ID: TRIM68
Family: Tripartite Motif

Synonyms: TRIM68, GC109, RNF137, Ro/SSA1 related protein, SS-56, Tripartite motif containing 68, RING finger protein 137, SS56, Tripartite motif-containing 68, SSA protein SS-56

ID gène: 55128

NCBI Accession: NP_060543

UniProt: Q6AZZ1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling