TRIM8 anticorps (AA 36-85)
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- Antigène Voir toutes TRIM8 Anticorps
- TRIM8 (Tripartite Motif Containing 8 (TRIM8))
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Épitope
- AA 36-85
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Reactivité
- Humain, Souris, Rat, Chien, Lapin, Boeuf (Vache), Porc, Poisson zèbre (Danio rerio), Singe, Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp TRIM8 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human TRIM8 / GERP
- Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse, Chicken, Xenopus (92%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic peptide located between aa36-85 of human TRIM8 (Q9BZR9, NP_112174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (100%), Horse, Turkey, Zebra finch, Chicken, Lizard, Xenopus (92%), Opossum (91%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product TRIM8 Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Antigène
- TRIM8 (Tripartite Motif Containing 8 (TRIM8))
- Autre désignation
- TRIM8 / GERP (TRIM8 Produits)
- Synonymes
- anticorps GERP, anticorps RNF27, anticorps AA408830, anticorps BB006755, anticorps Rnf27, anticorps si:ch211-208d15.5, anticorps wu:fc90g07, anticorps tripartite motif containing 8, anticorps tripartite motif containing 8 L homeolog, anticorps tripartite motif-containing 8, anticorps tripartite motif containing 8b, anticorps TRIM8, anticorps trim8.L, anticorps Trim8, anticorps trim8b
- Sujet
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Name/Gene ID: TRIM8
Family: Tripartite Motif
Synonyms: TRIM8, RING finger protein 27, Tripartite motif-containing 8, Tripartite motif containing 8, GERP, RNF27, Tripartite motif protein TRIM8 - ID gène
- 81603
- NCBI Accession
- NP_112174
- UniProt
- Q9BZR9
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