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TRIM8 anticorps (AA 36-85)

TRIM8 Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Porc, Poisson zèbre (Danio rerio), Hamster, Singe WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN6741584
  • Antigène Voir toutes TRIM8 Anticorps
    TRIM8 (Tripartite Motif Containing 8 (TRIM8))
    Épitope
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 36-85
    Reactivité
    • 35
    • 27
    • 20
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Porc, Poisson zèbre (Danio rerio), Hamster, Singe
    Hôte
    • 32
    • 2
    • 1
    Lapin
    Clonalité
    • 35
    Polyclonal
    Conjugué
    • 10
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TRIM8 est non-conjugé
    Application
    • 23
    • 13
    • 7
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human TRIM8 / GERP
    Homologie
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse, Chicken, Xenopus (92%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic peptide located between aa36-85 of human TRIM8 (Q9BZR9, NP_112174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (100%), Horse, Turkey, Zebra finch, Chicken, Lizard, Xenopus (92%), Opossum (91%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product TRIM8 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    Commentaires

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Conseil sur la manipulation
    Avoid repeat freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Antigène
    TRIM8 (Tripartite Motif Containing 8 (TRIM8))
    Autre désignation
    TRIM8 / GERP (TRIM8 Produits)
    Synonymes
    anticorps GERP, anticorps RNF27, anticorps AA408830, anticorps BB006755, anticorps Rnf27, anticorps si:ch211-208d15.5, anticorps wu:fc90g07, anticorps tripartite motif containing 8, anticorps tripartite motif containing 8 L homeolog, anticorps tripartite motif-containing 8, anticorps tripartite motif containing 8b, anticorps TRIM8, anticorps trim8.L, anticorps Trim8, anticorps trim8b
    Sujet
    Name/Gene ID: TRIM8
    Family: Tripartite Motif

    Synonyms: TRIM8, RING finger protein 27, Tripartite motif-containing 8, Tripartite motif containing 8, GERP, RNF27, Tripartite motif protein TRIM8
    ID gène
    81603
    NCBI Accession
    NP_112174
    UniProt
    Q9BZR9
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