PNPT1 anticorps (AA 489-538)
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- Antigène Voir toutes PNPT1 Anticorps
- PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
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Épitope
- AA 489-538
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Reactivité
- Humain, Rat, Chien, Cheval, Boeuf (Vache), Cobaye, Porc
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PNPT1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human PNPT1
- Homologie
- Percent identity by BLAST analysis: Human (100%) Mouse (92%) Rat, Guinea pig (85%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic peptide located between aa489-538 of human PNPT1 (Q8TCS8, NP_149100). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse (92%), Rat, Guinea pig (85%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product PNPT1 Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 50 μL sterile ddH2O.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Antigène
- PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
- Autre désignation
- PNPT1 (PNPT1 Produits)
- Synonymes
- anticorps COXPD13, anticorps DFNB70, anticorps OLD35, anticorps PNPASE, anticorps old-35, anticorps 1200003F12Rik, anticorps Old35, anticorps PNPase, anticorps Pnptl1, anticorps polyribonucleotide nucleotidyltransferase 1, mitochondrial, anticorps polyribonucleotide nucleotidyltransferase 1, anticorps CpipJ_CPIJ005886, anticorps PNPT1, anticorps Pnpt1
- Sujet
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Name/Gene ID: PNPT1
Synonyms: PNPT1, 3-5 RNA exonuclease OLD35, COXPD13, DFNB70, OLD35, PNPase 1, PNPase old-35, Old-35, PNPASE, Polynucleotide phosphorylase 1 - ID gène
- 87178
- NCBI Accession
- NP_149100
- UniProt
- Q8TCS8
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