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PNPT1 anticorps (AA 489-538)

PNPT1 Reactivité: Humain, Rat, Chien, Cheval, Boeuf (Vache), Cobaye, Porc WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN6741592
  • Antigène Voir toutes PNPT1 Anticorps
    PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
    Épitope
    • 15
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 489-538
    Reactivité
    • 41
    • 22
    • 18
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Rat, Chien, Cheval, Boeuf (Vache), Cobaye, Porc
    Hôte
    • 55
    • 3
    Lapin
    Clonalité
    • 57
    • 1
    Polyclonal
    Conjugué
    • 26
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PNPT1 est non-conjugé
    Application
    • 49
    • 21
    • 13
    • 13
    • 10
    • 9
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human PNPT1
    Homologie
    Percent identity by BLAST analysis: Human (100%) Mouse (92%) Rat, Guinea pig (85%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic peptide located between aa489-538 of human PNPT1 (Q8TCS8, NP_149100). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse (92%), Rat, Guinea pig (85%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product PNPT1 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    Commentaires

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute with 50 μL sterile ddH2O.
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Conseil sur la manipulation
    Avoid repeat freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Antigène
    PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
    Autre désignation
    PNPT1 (PNPT1 Produits)
    Synonymes
    anticorps COXPD13, anticorps DFNB70, anticorps OLD35, anticorps PNPASE, anticorps old-35, anticorps 1200003F12Rik, anticorps Old35, anticorps PNPase, anticorps Pnptl1, anticorps polyribonucleotide nucleotidyltransferase 1, mitochondrial, anticorps polyribonucleotide nucleotidyltransferase 1, anticorps CpipJ_CPIJ005886, anticorps PNPT1, anticorps Pnpt1
    Sujet
    Name/Gene ID: PNPT1

    Synonyms: PNPT1, 3-5 RNA exonuclease OLD35, COXPD13, DFNB70, OLD35, PNPase 1, PNPase old-35, Old-35, PNPASE, Polynucleotide phosphorylase 1
    ID gène
    87178
    NCBI Accession
    NP_149100
    UniProt
    Q8TCS8
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