ZNF777 anticorps (AA 468-517)

N° du produit ABIN6741624

Détail du produit anti-ZNF777 anticorps

Antigène: ZNF777 (Zinc Finger Protein 777 (ZNF777))

Épitope: AA 468-517

Reactivité: Humain, Souris, Rat, Cheval, Lapin, Boeuf (Vache), Cobaye, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ZNF777 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human ZNF777

Homologie: Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa468-517 of human ZNF777 (Q9ULD5, NP_056509). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Gorilla, Marmoset, Dog, Bat (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur ZNF777

Antigène: ZNF777 (Zinc Finger Protein 777 (ZNF777))

Autre désignation: ZNF777 (ZNF777 Produits)

Synonymes: anticorps 2500002G23Rik, anticorps ZNF777, anticorps RGD1566056, anticorps Znf777, anticorps zinc finger protein 777, anticorps Zfp777, anticorps ZNF777, anticorps LOC769049

Sujet: Name/Gene ID: ZNF777
Family: Zinc Finger

Synonyms: ZNF777, Zinc finger protein 777, KIAA1285

ID gène: 27153

NCBI Accession: NP_056509

UniProt: Q9ULD5