CYP11A1 anticorps (AA 288-337)
-
- Antigène Voir toutes CYP11A1 Anticorps
- CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))
-
Épitope
- AA 288-337
-
Reactivité
- Humain
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp CYP11A1 est non-conjugé
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human CYP11A1
- Homologie
- Percent identity by BLAST analysis: Human (100%).
- Purification
- Immunoaffinity purified
- Immunogène
-
Synthetic peptide located between aa288-337 of human CYP11A1 (P05108, NP_000772). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey (92%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product CYP11A1 Anticorps primaire
-
-
- Indications d'application
-
Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Commentaires
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Distilled Water.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
-
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
-
-
Steroidogenic enzyme Cyp11a1 regulates Type 2 CD8+ T cell skewing in allergic lung disease." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 20, pp. 8152-7, (2013) (PubMed).
: "
-
Steroidogenic enzyme Cyp11a1 regulates Type 2 CD8+ T cell skewing in allergic lung disease." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 20, pp. 8152-7, (2013) (PubMed).
-
- Antigène
- CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))
- Autre désignation
- P450SCC / CYP11A1 (CYP11A1 Produits)
- Synonymes
- anticorps CYP11A, anticorps CYP11A1, anticorps CYPXIA1, anticorps P450SCC, anticorps Cyp11a, anticorps Cypxia1, anticorps D9Ertd411e, anticorps P450scc, anticorps Scc, anticorps cscc, anticorps P450(scc), anticorps SSC, anticorps cytochrome P450 family 11 subfamily A member 1, anticorps cholesterol side-chain cleavage enzyme, mitochondrial-like, anticorps cytochrome P450 cholesterol side-chain cleavage, anticorps cytochrome P450, family 11, subfamily a, polypeptide 1, anticorps cytochrome P450, family 11, subfamily A, polypeptide 1, anticorps CYP11A1, anticorps LOC101827747, anticorps Cyp11a1
- Sujet
-
Name/Gene ID: CYP11A1
Family: Cytochrome P450
Synonyms: CYP11A1, Cholesterol 20-22 desmolase, Cholesterol desmolase, Cytochrome P450 11A1, Cytochrome P450C11A1, CYP11A, Cytochrome P450(scc), p450SCC, Steroid 20-22-lyase, CYPXIA1 - ID gène
- 1583
- NCBI Accession
- NP_000772
- UniProt
- P05108
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin
-