PGLS anticorps (C-Term)

N° du produit ABIN6744461

Détail du produit anti-PGLS anticorps

Antigène: PGLS (6-phosphogluconolactonase (PGLS))

Épitope: C-Term

Reactivité: Humain, Boeuf (Vache), Chien, Cheval, Porc, Roussette (Chauve-souris), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PGLS est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human PGLS

Homologie: Percent identity by BLAST analysis: Dog (100%) Mouse, Rat, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human PGLS (O95336, NP_036220). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Elephant, Dog, Bovine, Bat, Horse, Pig (100%), Gibbon, Mouse, Rat, Panda, Guinea pig (92%), Chicken, Platypus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur PGLS

Antigène: PGLS (6-phosphogluconolactonase (PGLS))

Autre désignation: PGLS / 6PGL (PGLS Produits)

Synonymes: anticorps 6PGL, anticorps PGLS, anticorps PSPTO1301, anticorps 1110030K05Rik, anticorps AI447866, anticorps Plgs, anticorps 6-phosphogluconolactonase, anticorps PGLS, anticorps pgl, anticorps CND03390, anticorps CNE04030, anticorps Tb11.02.4200, anticorps Pgls

Sujet: Name/Gene ID: PGLS

Synonyms: PGLS, 6PGL, 6-phosphogluconolactonase

ID gène: 25796

NCBI Accession: NP_036220

UniProt: O95336