Hexokinase 2 anticorps (AA 74-123)

N° du produit ABIN462257

Détail du produit anti-Hexokinase 2 anticorps

Antigène: Hexokinase 2 (HK2)

Épitope: AA 74-123

Reactivité: Humain, Souris, Rat, Porc, Chien, Cheval, Lapin, Boeuf (Vache), Singe, Cobaye, Poisson zèbre (Danio rerio), Hamster, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Hexokinase 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human HK2 / Hexokinase 2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Chicken, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa74-123 of human HK2 (P52789, NP_000180). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Sablefish, Stickleback (92%), Orangutan, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish, Sea squirt (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur Hexokinase 2

Antigène: Hexokinase 2 (HK2)

Autre désignation: HK2 / Hexokinase 2 (HK2 Produits)

Synonymes: anticorps HKII, anticorps HXK2, anticorps AI642394, anticorps hexokinase-2, anticorps fi09d05, anticorps fi17h06, anticorps wu:fi09d05, anticorps wu:fi17h06, anticorps wu:fi31b04, anticorps zgc:55926, anticorps HK2, anticorps ARABIDOPSIS THALIANA HEXOKINASE 2, anticorps ATHXK2, anticorps F6F22.11, anticorps F6F22_11, anticorps HEXOKINASE 2, anticorps hexokinase 2, anticorps StHK2, anticorps hexokinase 2, anticorps hexokinase-2, anticorps hexokinase 2 L homeolog, anticorps HK2, anticorps Hk2, anticorps hk2, anticorps HXK2, anticorps LOC100032849, anticorps hk2.L, anticorps hxk2, anticorps LOC102577690

Sujet: Name/Gene ID: HK2
Subfamily: Hexokinase
Family: Non-protein Kinase

Synonyms: HK2, Hexokinase II, Hexokinase type II, Hexokinase-2, muscle, HK II, HXK2, Hexokinase-2, Muscle form hexokinase, Hexokinase 2, HKII, Hxk 2

ID gène: 3099

NCBI Accession: NP_000180

UniProt: P52789

Pathways: Signalisation PI3K-Akt, Carbohydrate Homeostasis, L'effet Warburg