PIK3CA anticorps (AA 961-1068)

N° du produit ABIN677198

Détail du produit anti-PIK3CA anticorps

Antigène: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Épitope: AA 961-1068

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIK3CA est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Réactivité croisée: Boeuf (Vache), Humain, Souris, Lapin, Rat

Homologie: Cow,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human PI3KCA

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-PIK3CA (ABIN677198)

Paul-Samojedny, Suchanek, Borkowska, Pude?ko, Owczarek, Kowalczyk, Machnik, Fila-Dani?ow, Kowalski: "Knockdown of AKT3 (PKB?) and PI3KCA suppresses cell viability and proliferation and induces the apoptosis of glioblastoma multiforme T98G cells." dans: BioMed research international, Vol. 2014, pp. 768181, (2014) (PubMed).

Détails sur PIK3CA

Antigène: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Autre désignation: PI3KCA (PIK3CA Produits)

Synonymes: anticorps CLOVE, anticorps CWS5, anticorps MCAP, anticorps MCM, anticorps MCMTC, anticorps PI3K, anticorps p110-alpha, anticorps C-P3K, anticorps PI3-kinase, anticorps PI3K-alpha, anticorps pi3-k, anticorps pi3k, anticorps AA414921, anticorps C530050K14, anticorps p50alpha, anticorps p55alpha, anticorps p85alpha, anticorps 6330412C24Rik, anticorps caPI3K, anticorps p110, anticorps p110alpha, anticorps phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha, anticorps Phosphoinositide-3-kinase, catalytic, alpha polypeptide, anticorps phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha, anticorps phosphoinositide-3-kinase regulatory subunit 1, anticorps PIK3CA, anticorps GL50803_14855, anticorps pik3ca, anticorps Pik3ca, anticorps Pik3r1

Sujet:

Synonyms: MCM, CWS5, MCAP, PI3K, CLOVE, MCMTC, p11-alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 11 kDa catalytic subunit alpha, PtdIns-3-kinase subunit p11-alpha, p11alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PIK3CA

Background: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.

ID gène: 5290

UniProt: P42336

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling