FAK anticorps (pTyr397)

N° du produit ABIN685003

Détail du produit anti-FAK anticorps

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Épitope: pTyr397

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FAK est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This phosphorylation site is homologous to that of Tyr428 in Mouse and Tyr397 in Rat.

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH cunjugated synthetic phosphopeptide derived from human FAK 1 around the phosphorylation site of Tyr397

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-FAK (ABIN685003)

Yunes-Medina, Paciorkowski, Nuzbrokh, Johnson: "Depletion of transglutaminase 2 in neurons alters expression of extracellular matrix and signal transduction genes and compromises cell viability." dans: Molecular and cellular neurosciences, Vol. 86, pp. 72-80, (2018) (PubMed).

Détails sur FAK

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Autre désignation: FAK (PTK2 Produits)

Sujet:

Synonyms: FAK, FADK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, PTK2

Background: This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

ID gène: 5747

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling