LKB1 anticorps (pThr363)

N° du produit ABIN687877

Détail du produit anti-LKB1 anticorps

Antigène: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Épitope: pThr363

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LKB1 est non-conjugé

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain

Homologie: Mouse,Rat,Dog,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of Thr363 [IY(p-T)QD]

Isotype: IgG

Information d'application

Indications d'application: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur LKB1

Antigène: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Autre désignation: LKB1 (STK11 Produits)

Synonymes: anticorps pjs, anticorps LKB1, anticorps XEEK1, anticorps Stk11, anticorps PJS, anticorps hLKB1, anticorps AA408040, anticorps Lkb1, anticorps Par-4, anticorps R75140, anticorps mLKB1, anticorps wu:fj61a05, anticorps zgc:110180, anticorps xeek1, anticorps serine/threonine kinase 11, anticorps polarization-related protein LKB1, anticorps serine/threonine kinase 11 L homeolog, anticorps STK11, anticorps stk11, anticorps LOC662493, anticorps Stk11, anticorps stk11.L

Sujet:

Synonyms: PJS, LKB1, hLKB1, Serine/threonine-protein kinase STK11, Liver kinase B1, Renal carcinoma antigen NY-REN-19, STK11

Background: Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53, the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Isoform 2: Has a role in spermiogenesis.

ID gène: 6794

UniProt: Q15831

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg