CLTA/CLTB anticorps (N-Term)

N° du produit ABIN6937002

Détail du produit anti-CLTA/CLTB anticorps

Antigène: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Épitope: N-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CLTA/CLTB est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Purified recombinant N-terminal fragment of human Clathrin Light Chain

Clone: SPM174

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Placenta or Prostate Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CLTA/CLTB

Antigène: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Autre désignation: CLTA and CLTB (CLTA/CLTB Produits)

Synonymes: anticorps lca, anticorps zgc:73358, anticorps wu:fb61d08, anticorps wu:fj48d06, anticorps LCA, anticorps clc, anticorps DDBDRAFT_0169251, anticorps DDBDRAFT_0191305, anticorps DDB_0169251, anticorps DDB_0191305, anticorps clcb, anticorps clathrin light chain A, anticorps clathrin, light chain A, anticorps Clathrin light chain, anticorps clathrin light chain, anticorps clathrin light chain B, anticorps Clathrin light chain B, anticorps CLTA, anticorps clta, anticorps clic-1, anticorps CLC1, anticorps clc, anticorps CLTB, anticorps clcb, anticorps CNI00580, anticorps LOC5567495, anticorps CpipJ_CPIJ018056, anticorps PTRG_01988, anticorps PMAA_096950, anticorps SJAG_04517, anticorps TSTA_044110, anticorps PAS_chr1-4_0158

Sujet: Clathrin light chain A, Clathrin light chain B, Clathrin light chain LCA, Clathrin light chain LCB, Clathrin light polypeptide A, Clathrin light polypeptide B, Clathrin, light polypeptide (Lca), Clathrin, light polypeptide (Lcb), CLTA, CLTB,Clathrin, Light Chain
Cellular localisation: Cytoplasmic

Poids moléculaire: 31-44kDa

ID gène: 1211, 1212, 484241

UniProt: P09496, P09497