Keratin Basic anticorps

N° du produit ABIN6937077

Détail du produit

Antigène: Keratin Basic

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Specificité: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Purification: Purified by Protein A/G

Immunogène: Solubilized keratin extract from human stratum corneum

Clone: SPM591

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: PC12 cells, Skin, Prostate carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Buffer: 10 mM PBS without BSA and without Azide.

Agent conservateur: Azide free

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Date de péremption: 24 months

Détail du antigène

Antigène: Keratin Basic

Autre désignation: Cytokeratin, Basic (Type II or HMW) (Keratin Basic Produits)

Poids moléculaire: 67kDa (CK1), 58kDa (CK5), 56.5kDa (CK10), 50kDa (CK14)

ID gène: 3848, 3852, 3858

UniProt: P04264, P13647, P13645, P02533