FBXO5 anticorps (AA 1-250)

N° du produit ABIN6937217

Détail du produit anti-FBXO5 anticorps

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Épitope: AA 1-250

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FBXO5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: It recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Clone: EMI1-1176

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL for 60 minutes at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur FBXO5

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Autre désignation: FBXO5 (FBXO5 Produits)

Synonymes: anticorps EMI1, anticorps FBX5, anticorps Fbxo31, anticorps 2510044I10Rik, anticorps C85305, anticorps Emi1, anticorps emi1, anticorps fc65h02, anticorps wu:fc65h02, anticorps wu:fe06e07, anticorps wu:fz79f03, anticorps zgc:136397, anticorps zgc:158541, anticorps fbx5, anticorps Fbxo5, anticorps ACYPI007854, anticorps fbxo5, anticorps F-box protein 5, anticorps F-box only protein 5, anticorps F-box protein 5 L homeolog, anticorps F-box protein 5 S homeolog, anticorps FBXO5, anticorps Fbxo5, anticorps fbxo5, anticorps fbxo5.L, anticorps fbxo5.S

Sujet: Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31,EMI1 (Early Mitotic Inhibitor-1)
Cellular localisation: Nuclear

Poids moléculaire: 56kDa

ID gène: 26271, 520506

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases