MHC Class II HLA-DP/DQ/DR anticorps

N° du produit ABIN6937400

Détail du produit anti-MHC Class II HLA-DP/DQ/DR anticorps

Antigène: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC Class II HLA-DP/DQ/DR est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Coating (Coat), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Cells from human tonsil

Clone: CR3-43

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating use Ab at 2-4 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/ million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Raji cells. Tonsil, Spleen or lymph node.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MHC Class II HLA-DP/DQ/DR

Antigène: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Autre désignation: HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Produits)

Sujet: HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,HLA-DP/-DQ/-DR (MHC II)
Cellular localisation: Cell Surface

Poids moléculaire: 36kDa (' chain) and 27kDa (' chain)

ID gène: 3115, 3117, 3122

UniProt: P04440, P01908, P01909, P01920, P01903

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response