IGHG anticorps
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- Antigène Tous les produits IGHG
- IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp IGHG est non-conjugé
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Purified human Ig Gamma Chain
- Clone
- IG217
- Isotype
- IgG1 kappa
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- Indications d'application
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin/paraffin) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))
- Autre désignation
- IGHG (IGHG Produits)
- Synonymes
- anticorps AU044919, anticorps IGHG2A, anticorps Immunoglobulin heavy chain (gamma polypeptide), anticorps Ighg
- Sujet
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G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4,IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker)
Cellular localisation: Cytoplasm, Cell Surface and Secreted - Poids moléculaire
- 75kDa
- ID gène
- 3500, 3501, 3502
- UniProt
- P01857, P01859, P01860, P01861
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