IgL anticorps

N° du produit ABIN6937549

Détail du produit anti-IgL anticorps

Antigène: IgL

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IgL est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Purified IgG from human serum

Clone: N10-2

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur IgL

Antigène: IgL

Abstract: IgL Produits

Synonymes: anticorps IGL@, anticorps IGLC6, anticorps igl, anticorps immunoglobulin lambda locus, anticorps IGL, anticorps igl@

Sujet: Bence Jones Protein, BJP, IGLC 1/2/3, Mcg Marker, Paraprotein,Lambda Light Chain (B-Cell Marker)
Cellular localisation: Cell Surface, cytoplasmic and secreted

Poids moléculaire: ~22.5kDa

ID gène: 3535, 3546, 449585

UniProt: P01701, P01842