Keratin 5/8 anticorps
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- Antigène Tous les produits Keratin 5/8 (KRT5/8)
- Keratin 5/8 (KRT5/8)
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Keratin 5/8 est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
- Aucune reactivité croisée
- Poulet, Xenopus laevis
- Réactivité croisée (Details)
- Human. Pig. Mouse. Rat. Hamster. Cow. Dog. Sheep. Ferret. Does not react with Chicken or Xenopus.
- Immunogène
- Cytoskeletal preparation from HeLa cells
- Clone
- C-50
- Isotype
- IgG1 kappa
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- Indications d'application
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Agent conservateur
- Azide free
- Stock
- 4 °C
- Stockage commentaire
- Store at 2 to 8°C. Antibody is stable for 24 months.
- Date de péremption
- 24 months
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- Antigène
- Keratin 5/8 (KRT5/8)
- Autre désignation
- KRT5 & KRT8 (KRT5/8 Produits)
- Sujet
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CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 5/8
Cellular localisation: Cytoplasmic - Poids moléculaire
- 58kDa (CK5), 52.5kDa (CK8)
- ID gène
- 3852, 3856, 433845
- UniProt
- P13647, P05787
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