Cytokeratin 7 anticorps
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- Antigène Voir toutes Cytokeratin 7 (KRT7) Anticorps
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Cytokeratin 7 est non-conjugé
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
- Réactivité croisée (Details)
- Human. Rat.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant full-length human KRT7 protein (KRT7/760), OTN 11, ovarian carcinoma cell line (OV-TL12/30)
- Clone
- KRT7-760-OV-TL12-30
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product KRT7 Anticorps primaire
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- Indications d'application
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Carcinoma of ovary, lung, cervix or breast.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
- Autre désignation
- KRT7 (KRT7 Produits)
- Synonymes
- anticorps CK7, anticorps K2C7, anticorps K7, anticorps SCL, anticorps D15Wsu77e, anticorps Krt2-7, anticorps KRT7, anticorps keratin 7, anticorps KRT7, anticorps Krt7
- Sujet
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CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7,Cytokeratin 7 (Glandular and Transitional Epithelial Marker)
Cellular localisation: Cytoplasmic - Poids moléculaire
- 55kDa
- ID gène
- 3855, 411501
- UniProt
- P08729
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