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Keratin 77 anticorps

KRT77 Reactivité: Humain IHC, WB, IF, FACS, IHC (f) Hôte: Souris Monoclonal AE-1 unconjugated
N° du produit ABIN6937737
  • Antigène Voir toutes Keratin 77 (KRT77) Anticorps
    Keratin 77 (KRT77)
    Reactivité
    • 25
    • 7
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 18
    • 7
    Souris
    Clonalité
    • 16
    • 9
    Monoclonal
    Conjugué
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Keratin 77 est non-conjugé
    Application
    • 10
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificité
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
    Réactivité croisée (Details)
    Human. Monkey. Cow. Dog. Rabbit. Mouse. Rat. Chicken. Turtle.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogène
    Human epidermal keratin
    Clone
    AE-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product KRT77 Anticorps primaire
  • Indications d'application

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Skin or squamous cell carcinoma (IHC).

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Agent conservateur
    Azide free
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Date de péremption
    24 months
  • Antigène
    Keratin 77 (KRT77)
    Autre désignation
    KRT77 (KRT77 Produits)
    Synonymes
    anticorps K1B, anticorps KRT1B, anticorps 4732484G22Rik, anticorps K1emb, anticorps Krt239, anticorps mK1b, anticorps Kb39, anticorps keratin 77, anticorps KRT77, anticorps Krt77
    Sujet
    K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)
    Cellular localisation: Cytoplasmic
    Poids moléculaire
    40-56.5kDa
    ID gène
    374454, 334989
    UniProt
    Q7Z794
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