MTAP anticorps (AA 97-196)
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- Antigène Voir toutes MTAP Anticorps
- MTAP (Methylthioadenosine phosphorylase (MTAP))
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Épitope
- AA 97-196
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MTAP est non-conjugé
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Application
- Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)
- Clone
- MTAP-1813
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product MTAP Anticorps primaire
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- Indications d'application
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HepG2, A431, HeLa, HAP1 or MCF-7 cells. Kidney.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- MTAP (Methylthioadenosine phosphorylase (MTAP))
- Autre désignation
- MTAP (MTAP Produits)
- Synonymes
- anticorps BDMF, anticorps DMSFH, anticorps DMSMFH, anticorps LGMBF, anticorps MSAP, anticorps c86fus, anticorps Cc1-6, anticorps 1300019I21Rik, anticorps MTAPase, anticorps zgc:66012, anticorps mtap, anticorps methylthioadenosine phosphorylase, anticorps 5'-methylthioadenosine phosphorylase, anticorps 6-oxopurine nucleoside phosphorylase, anticorps methylthioadenosine phosphorylase L homeolog, anticorps MTAP, anticorps Mtap, anticorps mtaP, anticorps MMAH_RS04260, anticorps TAGG_RS05050, anticorps Toce_0364, anticorps VDIS_RS03165, anticorps MFER_RS02485, anticorps Intca_0886, anticorps DESMU_RS05370, anticorps Dester_1490, anticorps ARCVE_RS05445, anticorps Mahau_1873, anticorps MZHIL_RS01440, anticorps mtap, anticorps mtap.L
- Sujet
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BDMF, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL249, LGMBF, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, MSAP, MTA phosphorylase, MTAP, MTAPase, S-methyl-5''-thioadenosine phosphorylase,MTAP (Tumor Suppressor Marker)
Cellular localisation: Cytoplasmic - Poids moléculaire
- 31kDa
- ID gène
- 4507, 193268
- UniProt
- Q13126
- Pathways
- Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process
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