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TOP1MT anticorps

TOP1MT Reactivité: Humain IF, WB, FACS, IHC (f) Hôte: Souris Monoclonal TOP1MT-488 unconjugated
N° du produit ABIN6938633
  • Antigène Voir toutes TOP1MT Anticorps
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    Reactivité
    • 28
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 19
    • 11
    Souris
    Clonalité
    • 17
    • 13
    Monoclonal
    Conjugué
    • 14
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TOP1MT est non-conjugé
    Application
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 3
    • 2
    Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificité
    DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
    Réactivité croisée (Details)
    Human.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogène
    Recombinant full-length human TOP1MT protein
    Clone
    TOP1MT-488
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product TOP1MT Anticorps primaire
  • Indications d'application

    Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7 or A431 cells. Jurkat cell lysate. Human heart, skeletal muscle, brain or fetal liver tissue.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Agent conservateur
    Azide free
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Date de péremption
    24 months
  • Antigène
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    Autre désignation
    TOP1MT (TOP1MT Produits)
    Synonymes
    anticorps 2900052H09Rik, anticorps TOP1, anticorps topoisomerase (DNA) I, mitochondrial, anticorps DNA topoisomerase 1, mitochondrial, anticorps DNA topoisomerase I mitochondrial, anticorps TOP1MT, anticorps Top1mt
    Sujet
    DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase,Topoisomerase I, Mitochondrial (TOP1MT)
    Cellular localisation: Cytoplasm (Mitochondria)
    Poids moléculaire
    70kDa
    ID gène
    116447, 528574
    UniProt
    Q969P6
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