Occludin anticorps (AA 282-415)

N° du produit ABIN6939017

Détail du produit anti-Occludin anticorps

Antigène: Occludin (OCLN)

Épitope: AA 282-415

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Occludin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption.

Réactivité croisée (Details): Human,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

Clone: OCLN-2181

Isotype: IgG1

Information d'application

Indications d'application:

Positive Control: HepG2 cells. Kidney.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur Occludin

Antigène: Occludin (OCLN)

Autre désignation: OCLN (OCLN Produits)

Synonymes: anticorps AI503564, anticorps Ocl, anticorps ocln, anticorps oclnb, anticorps tpmt, anticorps OCLN, anticorps wu:fd23h10, anticorps wu:fi13c01, anticorps zgc:113992, anticorps zgc:56359, anticorps BLCPMG, anticorps occludin, anticorps occludin S homeolog, anticorps occludin a, anticorps Ocln, anticorps OCLN, anticorps ocln.S, anticorps ocln, anticorps oclna

Sujet: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PTORCH1, Tight junction protein occludin,Occludin (Marker of Early Blood Brain Barrier Damage)
Cellular localisation: Cell Surface and Cytoplasmic

Poids moléculaire: 60-82kDa

ID gène: 100506658, 592605

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C