CDKN1B anticorps

N° du produit ABIN6939047

Détail du produit anti-CDKN1B anticorps

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDKN1B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.

Réactivité croisée (Details): Human, Monkey, Mouse, Rat,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length Mouse p27 protein

Clone: DCS-72-F6

Isotype: IgG1

Information d'application

Indications d'application:

Positive Control: ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur CDKN1B

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: CDKN1B (CDKN1B Produits)

Synonymes: anticorps CDKN4, anticorps KIP1, anticorps MEN1B, anticorps MEN4, anticorps P27KIP1, anticorps cdkn1b, anticorps sb:cb611, anticorps wu:fb63g10, anticorps wu:fb64g10, anticorps wu:fe18e03, anticorps CDKN1B, anticorps Kip1, anticorps p27, anticorps FAM14D, anticorps ISG12, anticorps ISG12A, anticorps P27, anticorps Cdki1b, anticorps AA408329, anticorps AI843786, anticorps p27Kip1, anticorps cdkn1bl, anticorps cyclin dependent kinase inhibitor 1B, anticorps cyclin-dependent kinase inhibitor 1Bb, anticorps interferon alpha inducible protein 27, anticorps cyclin-dependent kinase inhibitor 1B, anticorps cyclin-dependent kinase inhibitor 1Ba, anticorps CDKN1B, anticorps cdkn1bb, anticorps IFI27, anticorps Cdkn1b, anticorps cdkn1ba

Sujet: CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4,p27Kip1 (Mitotic Inhibitor/Suppressor Protein)
Cellular localisation: Nuclear

Poids moléculaire: 25-26kDa

ID gène: 1027, 238990

UniProt: P46527

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy