CHD4 anticorps (C-Term)

N° du produit ABIN6939119

Détail du produit anti-CHD4 anticorps

Antigène: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Épitope: C-Term

Reactivité: Synthetic

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CHD4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Réactivité croisée (Details): Human, Mouse,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.

Clone: 3F2-4

Isotype: IgG2a

Information d'application

Indications d'application:

Positive Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur CHD4

Antigène: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Autre désignation: CHD4 (CHD4 Produits)

Synonymes: anticorps CHD4, anticorps im:7143343, anticorps si:ch211-51m24.3, anticorps si:ch211-57k11.10, anticorps wu:fb44b12, anticorps wu:fd12d03, anticorps MGC145363, anticorps Mi-2b, anticorps Mi2-BETA, anticorps Mi-2, anticorps 9530019N15Rik, anticorps AA617397, anticorps BC005710, anticorps D6Ertd380e, anticorps Mi-2beta, anticorps mKIAA4075, anticorps b230399n07, anticorps chromodomain helicase DNA binding protein 4, anticorps chromodomain helicase DNA binding protein 4a, anticorps chromodomain helicase DNA binding protein 4b, anticorps chromodomain helicase DNA binding protein 4 L homeolog, anticorps CHD4, anticorps chd4a, anticorps chd4b, anticorps chd4, anticorps Chd4, anticorps chd4.L

Sujet: ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta,CHD4
Cellular localisation: Nucleus and cytoplasm.

Poids moléculaire: 238kDa

ID gène: 1108, 162233

UniProt: Q14839