TOP1MT anticorps

N° du produit ABIN6939147

Détail du produit anti-TOP1MT anticorps

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TOP1MT est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human TOP1MT protein

Clone: TOP1MT-488

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: MCF-7, A431 cells. Jurkat cell lysate. Heart, Skeletal Muscle, Brain or Fetal Liver tissue.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TOP1MT

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Autre désignation: TOP1MT (TOP1MT Produits)

Synonymes: anticorps 2900052H09Rik, anticorps TOP1, anticorps topoisomerase (DNA) I, mitochondrial, anticorps DNA topoisomerase 1, mitochondrial, anticorps DNA topoisomerase I mitochondrial, anticorps TOP1MT, anticorps Top1mt

Sujet: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Poids moléculaire: 70kDa

ID gène: 116447

UniProt: Q969P6