Desmoglein 1 anticorps

N° du produit ABIN6939264

Détail du produit anti-Desmoglein 1 anticorps

Antigène: Desmoglein 1 (DSG1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmoglein 1 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificité: Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Purification: Purified by Protein A/G

Immunogène: Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain

Clone: DSG1-1733

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: A431 or A-375 cells. Skin.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Desmoglein 1

Antigène: Desmoglein 1 (DSG1)

Autre désignation: DSG1 (DSG1 Produits)

Synonymes: anticorps CDHF4, anticorps DG1, anticorps DSG, anticorps PPKS1, anticorps SPPK1, anticorps DSG-1, anticorps DSG1, anticorps Dsg1b, anticorps Dsg1c, anticorps desmoglein 1, anticorps desmoglein 4, anticorps DSG1, anticorps DSG4, anticorps Dsg1

Poids moléculaire: 150kDa (precursor), 160kDa (mature)

ID gène: 1828

UniProt: Q02413

Pathways: Cell-Cell Junction Organization