GAD anticorps (AA 72-135)
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- Antigène Voir toutes GAD (GAD1) Anticorps
- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
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Épitope
- AA 72-135
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp GAD est non-conjugé
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Coating (Coat)
- Specificité
- This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD's are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)
- Clone
- GAD1-2563
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product GAD1 Anticorps primaire
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- Indications d'application
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Positive Control: T98G or K-562 or HEK293 cells. Pancreas.
Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Western Blot (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
- Autre désignation
- GAD1 (GAD1 Produits)
- Synonymes
- anticorps CPSQ1, anticorps GAD, anticorps SCP, anticorps GAD-67, anticorps cpsq1, anticorps gad1, anticorps gad1-A, anticorps gad67, anticorps GAD67, anticorps GAD1, anticorps GLUTAMATE DECARBOXYLASE, anticorps GLUTAMATE DECARBOXYLASE 1, anticorps MKP11.30, anticorps MKP11_30, anticorps glutamate decarboxylase, anticorps EP10, anticorps GAD25, anticorps GAD44, anticorps Gad-1, anticorps Gad67, anticorps glutamate decarboxylase 1, anticorps glutamate decarboxylase 1 L homeolog, anticorps glutamate decarboxylase, anticorps glutamate decarboxylase 1b, anticorps GAD1, anticorps gad1.1.L, anticorps GAD, anticorps Gad1, anticorps gad1b
- Poids moléculaire
- 67kDa
- ID gène
- 2571
- UniProt
- Q99259
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