APC anticorps (AA 1-433)

N° du produit ABIN6939658

Détail du produit anti-APC anticorps

Antigène: APC (Adenomatous Polyposis Coli (APC))

Épitope: AA 1-433

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APC est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Aucune reactivité croisée: Souris

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment of human APC protein (around aa 1-433)

Clone: ALi 12-28

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur APC

Antigène: APC (Adenomatous Polyposis Coli (APC))

Autre désignation: APC (APC Produits)

Sujet: The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. Functions as a tumor suppressor, promoting rapid degradation of CTNNB1 and is a negative regulator of Wnt signaling. Also plays a role in HGF-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Poids moléculaire: 66kDa

ID gène: 324

UniProt: P25054

Pathways: Signalisation WNT, Stem Cell Maintenance, Cell-Cell Junction Organization