Recombinant TNC anticorps
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- Antigène Voir toutes TNC Anticorps
- TNC (Tenascin C (TNC))
- Type d'anticorp
- Recombinant Antibody
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp TNC est non-conjugé
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Application
- Immunohistochemistry (IHC), Staining Methods (StM)
- Specificité
- In Western blotting, it reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
- Aucune reactivité croisée
- Rat (Rattus)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant human full-length TNC protein
- Clone
- TNC-2981R
- Isotype
- IgG
- Top Product
- Discover our top product TNC Anticorps primaire
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- Indications d'application
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Positive Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.
Known Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) ,Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- TNC (Tenascin C (TNC))
- Autre désignation
- TNC (TNC Produits)
- Poids moléculaire
- 210kDa and 300kDa
- ID gène
- 3371
- UniProt
- P24821
- Pathways
- Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
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