Cytokeratin 7 anticorps
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- Antigène Voir toutes Cytokeratin 7 (KRT7) Anticorps
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Cytokeratin 7 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM), Immunostaining (ISt)
- Specificité
- It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
- Immunogène
- Recombinant full-length human KRT7 protein
- Clone
- KRT7-1499R
- Isotype
- IgG
- Top Product
- Discover our top product KRT7 Anticorps primaire
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- Indications d'application
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Positive Control: HeLa cells. Carcinoma of ovary, lung, cervix or breast.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
- Autre désignation
- KRT7 (KRT7 Produits)
- Synonymes
- anticorps CK7, anticorps K2C7, anticorps K7, anticorps SCL, anticorps D15Wsu77e, anticorps Krt2-7, anticorps KRT7, anticorps keratin 7, anticorps KRT7, anticorps Krt7
- Poids moléculaire
- 55kDa
- ID gène
- 3855
- UniProt
- P08729
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