EpCAM anticorps (Extracellular Domain)
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- Antigène Voir toutes EpCAM (EPCAM) Anticorps
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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Épitope
- AA 77-202, Extracellular Domain
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp EpCAM est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant human EpCAM fragment from extracellular domain (around aa 77-202) (exact sequence is proprietary)
- Clone
- EGP40-1372
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product EPCAM Anticorps primaire
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- Indications d'application
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Positive Control: MCF-7, HT29 cells. Ovarian carcinoma.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Autre désignation
- TACSTD1 (EPCAM Produits)
- Sujet
- EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
- Poids moléculaire
- 40-43kDa
- ID gène
- 4072
- UniProt
- P16422
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