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EpCAM anticorps (Extracellular Domain)

Name: EpCAM anticorps (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN6939977
Price: 663.89 EUR
Availability: InStock

EPCAM Reactivité: Humain WB, FACS, IF, IHC, StM Hôte: Souris Monoclonal EGP40-1372 unconjugated

Formalin-fixed, paraffin-embedded human Colorectal Carcinoma stained with EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM anticorps (Extracellular Domain))

IHC

Western Blot of human lung lysate using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM anticorps (Extracellular Domain))

Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (EpCAM anticorps (Extracellular Domain))

PAr

Confocal Immunofluorescence of human Colo-rectal Carcinoma. (EpCAM anticorps (Extracellular Domain))

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (EpCAM anticorps (Extracellular Domain))

FACS

Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM anticorps (Extracellular Domain))

IHC

Immunofluorescent staining of MCF-7 cells. (EpCAM anticorps (Extracellular Domain))

SDS-PAGE Analysis Purified EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM anticorps (Extracellular Domain))

SDS

Western Blot of 293, A431 and HCT116 cell lysate using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM anticorps (Extracellular Domain))

9 images

N° du produit ABIN6939977

Fiche de données

Antigène Voir toutes EpCAM (EPCAM) Anticorps

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
Épitope
56

41

30

29

16

16

14

13

12

11

10

8

5

4

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 77-202, Extracellular Domain
Reactivité
308

87

36

2

1

1
Humain
Hôte
162

153

20

1
Souris
Anticorps secondaires appropriés
Clonalité
215

117

2
Monoclonal
Conjugué
163

22

19

10

9

9

8

8

8

8

8

5

5

5

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
Cet anticorp EpCAM est non-conjugé
Application
220

192

124

116

90

38

30

26

24

21

16

16

15

11

4

4

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Purification

Purified by Protein A/G

Immunogène

Recombinant human EpCAM fragment from extracellular domain (around aa 77-202) (exact sequence is proprietary)

Clone

EGP40-1372

Isotype

IgG1 kappa

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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reactivité: Humain WB, IHC, StM Hôte: Souris Monoclonal rEGP40-1372 unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibody
EPCAM Reactivité: Humain WB, FACS, IHC, ELISA Hôte: Souris Monoclonal 7B5C10 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reactivité: Humain FACS, IF, IHC, ELISA, StM, Coat Hôte: Souris Monoclonal EGP40-1384 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reactivité: Humain WB, FACS, IF, IHC, StM Hôte: Souris Monoclonal EGP40-1373 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibody
EPCAM Reactivité: Humain FACS, IHC, StM Hôte: Lapin Monoclonal EGP40-1555R unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibody
EPCAM Reactivité: Humain, Souris, Rat IHC, StM Hôte: Souris Monoclonal EGP40-1110 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) antibody
EPCAM Reactivité: Humain WB, FACS, IHC (p), IP, ICC Hôte: Souris Monoclonal 323-A3 unconjugated

Indications d'application

Positive Control: MCF-7, HT29 cells. Ovarian carcinoma.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-80 °C

Stockage commentaire

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption

24 months
Antigène

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation

TACSTD1 (EPCAM Produits)

Sujet

EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Poids moléculaire

40-43kDa

ID gène

4072

UniProt

P16422