Recombinant EpCAM anticorps (Extracellular Domain)
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- Antigène Voir toutes EpCAM (EPCAM) Anticorps
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Type d'anticorp
- Recombinant Antibody
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Épitope
- AA 100-224, Extracellular Domain
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp EpCAM est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- Specificité
- Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
- Immunogène
- Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)
- Clone
- EGP40-2041R
- Isotype
- IgG
- Top Product
- Discover our top product EPCAM Anticorps primaire
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- Indications d'application
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Positive Control: MCF-7 or HT29 cells. Breast tumor.
Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Autre désignation
- TACSTD1 (EPCAM Produits)
- Poids moléculaire
- 40-43kDa
- ID gène
- 4072
- UniProt
- P16422
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