MLH1 anticorps

N° du produit ABIN6940057

Détail du produit anti-MLH1 anticorps

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLH1 est non-conjugé

Application: ELISA, Coating (Coat)

Specificité: This MAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.

Purification: Purified by Protein A/G

Immunogène: Recombinant human MLH1 protein

Clone: MLH1-1324

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: Molt-4, HeLa or Jurkat cells. Lymph node or Colon, breast, lung, testis, prostate or thyroid carcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MLH1 (MLH1 Produits)

Synonymes: anticorps CG11482, anticorps Dmel\\CG11482, anticorps dmlh-1, anticorps dmlh1, anticorps zgc:66301, anticorps MLH1, anticorps LOC100232198, anticorps 1110035C23Rik, anticorps AI317206, anticorps AI325952, anticorps AI561766, anticorps COCA2, anticorps FCC2, anticorps HNPCC, anticorps HNPCC2, anticorps hMLH1, anticorps mutL homolog 1, anticorps CG11482 gene product from transcript CG11482-RB, anticorps mutL homolog 1 S homeolog, anticorps mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), anticorps DNA mismatch repair protein Mlh1, anticorps MLH1, anticorps Mlh1, anticorps mlh1.S, anticorps mlh1, anticorps LOC588545

Poids moléculaire: 85kDa

ID gène: 4292

UniProt: P40692

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response