CD73 anticorps
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- Antigène Voir toutes CD73 (NT5E) Anticorps
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD73 est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant full length human NT5E protein
- Clone
- NT5E-2503
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product NT5E Anticorps primaire
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- Indications d'application
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Positive Control: Tonsil, Prostate or Lung Carcinoma.
Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Autre désignation
- NT5E (NT5E Produits)
- Synonymes
- anticorps CALJA, anticorps CD73, anticorps E5NT, anticorps NT, anticorps NT5, anticorps NTE, anticorps eN, anticorps eNT, anticorps zgc:63784, anticorps NT5E, anticorps MGC114869, anticorps PSPTO2277, anticorps 2210401F01Rik, anticorps AI447961, anticorps Nt5, anticorps 5'-nucleotidase ecto, anticorps 5'-nucleotidase, ecto (CD73), anticorps 5'-nucleotidase, anticorps 5' nucleotidase, ecto, anticorps NT5E, anticorps nt5e, anticorps nt5e.S, anticorps PSPTO_2277, anticorps CBU_1233, anticorps MCA1322, anticorps Nt5e
- Sujet
- CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
- Poids moléculaire
- 71kDa
- ID gène
- 4907
- UniProt
- P21589
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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